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KMID : 0545120050150051115
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 5 p.1115 ~ p.1119
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Improvement of Photoheterotrophic Hydrogen Production of Rhodobacter sphaeroides by Removal of B800-850 Light-Harvesting Complex
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Kim EJ
Yoo SB/Kim MS/Lee JK
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Abstract
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The photoheterotrophic H2 production of Rhodobacter sphaeroides was significantly increased through disruption of the genes coding for uptake hydrogenase and poly-β- hydroxybutyrate (PHB) synthase (Lee et al., Appl. Microbiol. Biotechnol. 60: 147-153, 2002). In this work, we further removed the B800-850 light-harvesting (LH) complex from the strain and found an increase in H2 production at the lightsaturating cell growth (≥10Watts [W]/m2). Neither the mutant nor the wild-type produced more H2 at the brighter light. Accordingly, light does not appear to be limited for the H2 production by the presence of B800-850. However, increase in the level of the spectral complexes resulted in decrease of H2 production. Thus, although the B875 is essential for light harvesting, the consumption of cellular energy for the synthesis of B800-850 and the surplus LH complexes may reduce the energy flow into the H2 production of R. sphaeroides.
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